Detailed Notes on 지방이식
Detailed Notes on 지방이식
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Do you do have a protocol or recommended antibodies to execute ICC staining on my completely differentiated ALI cultures?
As soon as the operate is complete, retrieve the Delivery Cartridge in the instrument. The cell sample must be at the bottom or aspect of the collection tube. Be aware: It is recommended to spin down the Shipping and delivery Cartridge within a mini-centrifuge for any handful of seconds for complete volume Restoration.
If making use of Gentle Cell Dissociation Reagent (GCDR), the length of GCDR therapy must be optimized. Incubate organoids with GCDR for 8 -ten minutes at 37°C and use a P1000 pipette to Carefully pipette up and down, and visually inspect if organoids split up.
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Meticulously take out the supernatant without having disturbing the organoid pellet. Insert 0.5 mL of sought after single-cell dissociation reagent to your tube. Certain incubation suggestions for various dissociation reagents are observed beneath:
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There are several commercially obtainable resources for Principal airway epithelial cells. We've analyzed HAECs from two distributors, Lonza and Epithelix — both equally have resulted in productive ALI cultures.
Note: Carry on to antibody staining if accomplishing Reside cell Examination just after this action. Alternatively, carry on to fixable dye staining and PFA fixation.
In case cell clumping is observed just after thawing cryopreserved cells, it is recommended to filter aggregated suspensions via a 37 µm cell strainer (e.g. 자가지방이식 Catalog #27250) for ideal benefits. In cases like this, the operate time on the CellPore™ Transfection System may well need to be enhanced to ten seconds.
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